Journal: Acta pharmacologica Sinica

Article Title: Role of hypothalamus nociceptin/orphanin FQ in pre-ovulatory luteinizing hormone surge of estrogen and progesterone-primed, ovariectomized rats.

doi: 10.1111/j.1745-7254.2007.00587.x

Figure Lengend Snippet: Figure 3. RT-PCR analysis for OFQ mRNA levels in POA of cycling female rats during the estrous cycle. The upper lanes are β-actin (420 bp, as internal control) and the lower lanes are OFQ (255 bp). (M: Marker, A: Diestrus, B: Proestrus, C: Estrus)

Article Snippet: The polyclonal antibodies against the ORL1 receptor (1:500, Santa Cruz Bio-technology, Santa Cruz, CA, USA) and β-actin (1:500, Boster Biotechnology Company, Wuhan, China) were used to detect changes of the ORL1 receptor protein and β-actin (as the internal control) according to the manufacturer’s protocols.

Techniques: Reverse Transcription Polymerase Chain Reaction, Control, Marker

Journal: Acta pharmacologica Sinica

Article Title: Role of hypothalamus nociceptin/orphanin FQ in pre-ovulatory luteinizing hormone surge of estrogen and progesterone-primed, ovariectomized rats.

doi: 10.1111/j.1745-7254.2007.00587.x

Figure Lengend Snippet: Figure 4. Semiquantitative RT-PCR analysis for expression of OFQ and ORL1 receptor mRNA levels in POA during the estrous cycle. The data were expressed as relative values of mean optic density and the three black columns represent the [OFQ]/[β-actin] mRNA ratios on diestrus, proestrus and estrus, respectively. Accordingly, [ORL1 receptor]/[β-actin] mRNA ratios in the three stages are expressed in three white columns. n=6. Mean±SD. bP<0.05 vs Diestrus.

Article Snippet: The polyclonal antibodies against the ORL1 receptor (1:500, Santa Cruz Bio-technology, Santa Cruz, CA, USA) and β-actin (1:500, Boster Biotechnology Company, Wuhan, China) were used to detect changes of the ORL1 receptor protein and β-actin (as the internal control) according to the manufacturer’s protocols.

Techniques: Reverse Transcription Polymerase Chain Reaction, Expressing

Journal: Acta pharmacologica Sinica

Article Title: Role of hypothalamus nociceptin/orphanin FQ in pre-ovulatory luteinizing hormone surge of estrogen and progesterone-primed, ovariectomized rats.

doi: 10.1111/j.1745-7254.2007.00587.x

Figure Lengend Snippet: Figure 9. Western blot analysis of ORL1 receptor protein levels in the medial basal hypothalamus (MBH) of the adult cycling rat. The data were expressed as relative values of mean optic density and the bars represent [ORL1 receptor]/[β-actin] protein ratios (A: Diestrus, B: Proestrus, C: Estrus). n=6. Mean±SD. bP<0.05 vs control.

Article Snippet: The polyclonal antibodies against the ORL1 receptor (1:500, Santa Cruz Bio-technology, Santa Cruz, CA, USA) and β-actin (1:500, Boster Biotechnology Company, Wuhan, China) were used to detect changes of the ORL1 receptor protein and β-actin (as the internal control) according to the manufacturer’s protocols.

Techniques: Western Blot, Control

Journal: Journal of Neuroscience Research

Article Title: Neuronal extracellular microRNAs miR‐124 and miR‐9 mediate cell–cell communication between neurons and microglia

doi: 10.1002/jnr.24344

Figure Lengend Snippet: Antibody characterization

Article Snippet: Rabbit polyclonal antibodies for human/mouse/rat HDLs (immunogen: KLH conjugated synthetic peptide from a 41–125 amino acid sequence of human HDL) were purchased from Biorbyt (cat#orb1003; 0.5 mg/ml, dilution 1:1,000; RRID: AB_2737031).

Techniques: Concentration Assay, Marker, Binding Assay, Immunofluorescence, Blocking Assay, Sequencing, Western Blot